Journal: Scientific Reports

Article Title: Screening of key genes related to disulfidptosis in psoriasis based on the analysis of WGCNA

doi: 10.1038/s41598-025-28457-w

Figure Lengend Snippet: A-F:Box plot of target gene expression differences.The p values of all genes were less than 0.05, but the expression levels of SLC7A11 ( A ), S100A12 ( B ), GYS1 ( C ) and FRK ( D ) genes in the experimental group were higher than those in the control group; and the expression levels of MYH10 ( E ) and HECW2 ( F ) genes in the experimental group were lower than those in the control group.

Article Snippet: The primary antibodies used included GYS1 polyclonal antibody (1:2000, Cat. 10,566–1-AP, Proteintech), S100A12 polyclonal antibody (1:2000, Cat. 16,630–1-AP, Proteintech), and SLC7A11 polyclonal antibody (1:2000, Cat. 26,864–1-AP, Proteintech). (1:500–1:2000), incubated at 4°C overnight; the secondary antibody was HRP-labeled goat anti-rabbit IgG (1:15,000, GB23303, Seville Biotech) incubated at room temperature for 1 h, and the chemiluminescence method was used for imaging, with the gray value of the band analyzed using Image Lab software and normalized with the internal reference GAPDH.

Techniques: Targeted Gene Expression, Expressing, Control

Journal: Scientific Reports

Article Title: Screening of key genes related to disulfidptosis in psoriasis based on the analysis of WGCNA

doi: 10.1038/s41598-025-28457-w

Figure Lengend Snippet: F:Gene ROC curve. SLC7A11 ( A ) and S100A12 ( B ) are potential ideal biomarkers, followed by GYS1 ( C ) and FRK ( D ), and MYH10 ( E ) and HECW2 ( F ) can be used as complementary indicators.

Article Snippet: The primary antibodies used included GYS1 polyclonal antibody (1:2000, Cat. 10,566–1-AP, Proteintech), S100A12 polyclonal antibody (1:2000, Cat. 16,630–1-AP, Proteintech), and SLC7A11 polyclonal antibody (1:2000, Cat. 26,864–1-AP, Proteintech). (1:500–1:2000), incubated at 4°C overnight; the secondary antibody was HRP-labeled goat anti-rabbit IgG (1:15,000, GB23303, Seville Biotech) incubated at room temperature for 1 h, and the chemiluminescence method was used for imaging, with the gray value of the band analyzed using Image Lab software and normalized with the internal reference GAPDH.

Techniques: